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KMID : 0881720180330050354
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Journal of Food Hygiene and Safety
2018 Volume.33 No. 5 p.354 ~ p.360
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Method Development for Determination of Trichothecene Mycotoxins in Nuts by LC-MS/MS
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Kim Dan-Bi
Park Ji-Su
Yoo Mi-young
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Abstract
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This study presents a method validation for extraction and quantitative analysis of trichothecene mycotoxins in nuts based on the quick, easy, cheap, effective, rugged, and safe (QuEChERS) approach for extraction and enhanced matrix removal (EMR)-lipid-disperive-SPE (d-SPE) cleanup method, with detection and quantification by high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in positive- and negative-ion modes. Linearity, precision, and accuracy were validated for the LC-MS/MS methods. Results obtained with the LC-MS/MS were linear, with a correlation coefficient (R2) of 0.998. Limits of detection and quantification for mycotoxins were 0.41 to 3.57 μg/kg and 1.23 to 10.82 μg/kg, respectively. Intra- and inter-day precisions (RSD, %) were 0.40 to 8.44% and 1.93 to 12.46%, respectively. Results indicated to be rapidly and accurately identifying trichothecene mycotoxins and may be used as a suitable safety management method for nuts and nuts related commodities.
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KEYWORD
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Trichothecene mycotoxins, Nuts, QuEChERS, LC-MS/MS, Analytical method
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